Costa Rica face Spain on 23 November, followed by Japan, then Germany, in Group E.
They became the last team to qualify after beating New Zealand in an intercontinental play-off in June.
The country did not progress beyond the group stage at the 2018 World Cup, but reached the quarter-finals in 2014.
It was very difficult [to choose the squad] because there are not only 26 players eligible to be in Qatar. We asked ourselves more questions about what we needed for the games and the current situation of each of themhead coach Luis Fernando Suarez
Costa Rica Squad
Goalkeepers: Keylor Navas (Paris St-Germain), Esteban Alvarado (Herediano), Patrick Sequeira (CD Lugo)
Defenders: Francisco Calvo (Konyaspor), Juan Pablo Vargas(Millonarios FC), Kendall Waston (Saprissa), Oscar Duarte (Al-Wehda), Daniel Chacon (Colorado Rapids), Keysher Fuller (Herediano), Carlos Martinez (San Carlos), Bryan Oviedo (Real Salt Lake), Ronald Matarrita (Cincinnati)
Midfielders: Yeltsin Tejeda (Herediano), Celso Borges (Alajuelense), Youstin Salas (Saprissa), Roan Wilson (Grecia), Gerson Torres (Herediano), Douglas Lopez (Herediano) Jewisson Bennette (Sunderland), Alvaro Zamora (Saprissa), Anthony Hernandez (Puntarenas FC), Brandon Aguilera (Nottingham Forest), Bryan Ruiz (Alajuelense)
Forwards: Joel Campbell (Leon), Anthony Contreras (Herediano) Johan Venegas (Alajuelense)